Question 1

How do you make this nutrient rich agar we used it as is and nothing grew daughter project results are due next week please help we have a another 15 gram packet how much beef bouillon and sugar do I need to add for 1liter prep
Thanks

Accepted Answer

Nothing grew in this agar until we made batches with nutrients. Since we broke the 15g down into smaller lots, we ended up adding nutrients by weight. Most folks don't have a scale for this, so my best guess would be 1 Tablespoon of beef or chicken bullion plus 1 Tablespoon of sugar for the entire 15g package. If all else fails, you can substitute plain Knox gelatin for the agar. But, gelatin is not stable at room temperature and especially not in an incubator (it melts after a while into goo). You'll be forced to leave the Knox gelatin experiment in the fridge. Even in that cold environment, some bacteria will grow.

Question 2

What is the shelf life of the pocket open and unopened? Should I I store this with desiccant packs?

Accepted Answer

I’ve had the first packet for close to a year and half without any issues after opening it. I would rather put a box of baking soda by the bag rather than put desiccant packets in there. I only say this because so much as sticking your hands in there can potentially compromise the integrity of the product.

Question 3

Is this good for making a salt bridge?

Accepted Answer

Hello, yes, this is good for making a salt bridge.

Question 4

My bacteria is not growing. I have used 12 sterilized Petrie dishes and distilled water. It has been several days. Why is this medium not working?

Accepted Answer

This product is only agar- it does not have the nutrients that microorganisms need to grow. This product is used to make water set up as a gel but you will need to add some beef boullion & sugar (or other nutrients if you are looking for specific bacteria) if you want to grow microorganisms. You could also purchase a product called NUTRIENT AGAR which already has the nutrients mixed with it.

ASIN	B008SYV5VI
Best Sellers Rank	#494,810 in Industrial & Scientific ( See Top 100 in Industrial & Scientific ) #36 in Lab Natural Proteins
Brand	The Seaweed Solution
Brand Name	The Seaweed Solution
Coin Variety 1	Agar Agar
Cuisine	Versatile
Customer Reviews	4.2 out of 5 stars 213 Reviews
Item Form	Powder
Item Package Weight	0.02 Kilograms
Item Weight	22.68 g
Manufacturer	USA
Material Feature	Natural, Organic, Vegan
Material Features	Natural, Organic, Vegan
Number of Items	1
Number of Pieces	15
Package Weight	0.02 Kilograms
Part Number	1O-VY2X-QIEI
Size	0.53 Ounce (Pack of 1)
UPC	609408017186 885844095296
Variety	Agar Agar

Agar Agar Powder - 15 grams, Laboratory Grade

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