*Specificity: This assay has high sensitivity and excellent specificity for detection of Monkey FST. No significant cross-reactivity or interference between Monkey FST and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Monkey FST and all the analogues, therefore, cross reaction may still exist.Sample Type: Serum, Plasma, Biological Fluids; *Accession#: NP_037541.1; NM_013409.2; P19883; *Description: Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FST in Monkey serum, plasma and other biological fluidsPrinciple of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey FST. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Monkey FST and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey FST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of alpha1-AGP. You can calculate the concentration of Monkey FST in the samples by comparing the OD of the samples to the standard curve.; *NCBI Summary: Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of...
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